1Department of Neurology, Medical School of National and Kapodistrian University of Athens, Aeginition Hospital, Athens 11528, Greece.
2Department of Neurology, Immunogenetics Laboratory and Division of Demyelinating Diseases, Medical School of National and Kapodistrian University of Athens, Aeginition Hospital, Athens 11528, Greece.
Correspondence Address: Prof. Maria Constantinos Anagnostouli, Department of Neurology, Immunogenetics Laboratory and Division of Demyelinating Diseases, Medical School of National and Kapodistrian University of Athens, Aeginition Hospital, Vassilisis Sofias Avenue 72-4, Athens 11528, Greece. E-mail: email@example.com
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License (http://creativecommons.org/licenses/by-nc-sa/3.0/), which allows others to remix, tweak and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
Neuromyelitis optica (NMO) is an autoimmune demyelinating disorder, predominantly characterized by severe optic neuritis, transverse myelitis and the high level of antibodies against aquaporin-4 (AQP4) or NMO‑immunoglobulin G (IgG). Researches trying to correlate NMO with specific human leukocyte antigen (HLA) alleles took place in a limited extend in the last few years. Nevertheless, it has become clear that HLAs play a crucial role in the genetic risk of NMO, in the understanding of its pathogenesis and the differential diagnosis mainly from multiple sclerosis (MS), and also from other demyelinating diseases. In this study, we retrieved all the available data in the MEDLINE concerning the distribution of HLA frequencies in NMO and NMO‑spectrum diseases, in all available ethnic groups, and compared them with those of MS. The results suggest that, the well‑established HLA-DRB1*15:01 allele, associated with MS, plays rather a protective role for NMO. HLA-DRB1*03 allele is highly frequent in the NMO-IgG positive Caucasian patients, while HLA-DPB1*05:01 is the predominant allele in Japanese patients. The HLA-genotype and anti-AQP4 presence are the common immunological components in cases of comorbidity of NMO and other autoimmune diseases. The authors aim to summarize in the critical review the results of these researches worldwide, create a workable table including all this information for an easier reading approach and highlight the importance of these results in therapeutic decision making, using the HLA profile as biomarker in patients’ stratification.
Diagnosis, human leukocyte antigens‑immunogenetics, immunopathogenesis, neuromyelitis optica, treatment
Since its very first discovery and disease association studies in early 1970’s, the major histocompability complex (MHC) with its polymorphisms has been the “gold standard” and the primer genetic locus in attributing genetic burden for certain autoimmune diseases, like multiple sclerosis (MS). Initial studies, using serological techniques, showed an association of MS with human leukocyte antigens (HLA) class I, especially HLA-A3 and HLA-B7. Multiple recent researches which used current molecular methods [sequence specific oligonucleotide-polymerase chain reaction (PCR), single specific primer-PCR and single-nucleotide polymorphisms, genome-wide association study, etc.] and which were conducted in many MS cohorts, made clear that HLA-DRB1*15:01 is by far the main independent, responsible allele for attributing genetic risk in different MS ethnic groups. In addition, co-existence of certain alleles probably leads to an increase or decrease of the overall risk, via epistatic mechanisms (i.e. HLA-DRB1*15:01 and HLA-DQ1*01:02). Moreover, a Vitamin D response element has been found in the promoter region of HLA-DRB1*15:01, changing the expression of the allele and the risk for the disease. Thus, an environmental factor, the sunlight, via the metabolites of Vitamin D, has been linked to the genome, especially to HLA-DRB1*15:01 and finally to the disease phenotype. The interaction between HLA-DRB1*15:01 and Epstein-Barr virus, as well as the estrogen receptor, has also been very well established.
Neuromyelitis optica (NMO) is an autoimmune demyelinating disorder, predominantly characterized by severe optic neuritis (ON) and transverse myelitis (TM). It was considered as a variant of MS, but the discovery that most NMO patients have antibodies against aquaporin-4 (AQP4) or NMO-immunoglobulin G (IgG), dramatically changed our perception of the disease and brought NMO and its spectrum in the center of interest. Researches trying to correlate NMO with specific HLA alleles took place in a limited extend in the last few years, especially in Japanese population, in which NMO appears its greatest frequency. Nevertheless, it has become clear that HLA play a crucial, and maybe the primer role, in the genetic risk of NMO and provide great insight in the profound understanding of its pathogenesis and the differential diagnosis mainly from MS and other demyelinating diseases as well.
In this study, our aim was to summarize in a critical review the results of these researches worldwide and shed light on the contribution of HLA alleles in NMO immunopathogenesis, given the total absence of such a review.
Neuromyelitis optica was first described in 1870 by Allbutt, who reported an association between myelitis and unilateral optic nerve disorder, but it was in 1894 when the term “neuromyelite optique aigue” (acute optic neuromyelitis) was coined by Devic in order to describe patients who first suffered unilateral or bilateral loss of vision and within weeks developed severe spastic para- or tetraparesis, loss of sensation and sphincter control. In 1999, Wingerchuk et al. proposed the first diagnostic criteria for NMO. Current revised criteria for diagnosing NMO were defined in 2006 by the same group [Table 1], because in 2004, the AQP4 protein was identified for NMO, which was the first molecular target described for any type of demyelinating diseases of the central nervous system (CNS).
Revised diagnostic criteria for NMO
|At least two of three supportive criteria|
|1. Contiguous spinal cord MRI lesion extending over three vertebral segments|
|2. Brain MRI not meeting diagnostic criteria for multiple sclerosis|
|3. NMO-IgG seropositive status|
Neuromyelitis optica represents < 1% of demyelinating diseases of the CNS in Caucasians and it is certainly more common in Asians. It has been reported to account for up to 30% of West Indian cases of CNS demyelination, 20-30% of Japanese cases, 48% of East Asian cases, 23% of Indian cases and 15% of Afro-Brazilian cases. Japanese patients with opticospinal MS (OSMS) or Asian MS represent a distinct entity from western MS. The equal detection of NMO-IgG in the sera of Japanese patients with OSMS and NMO, as well as the similar clinical and pathological characteristics, indicate that both syndromes may belong to the same clinical entity. NMO is more common in women than men (> 2/3).[17,18] More than 80% present the relapsing form of the disease, while the median age of onset is the late 30s, with few reports of NMO occurrence in children or elderly. Familial cases of NMO are estimated to account for 3% of all NMO cases.
Neuromyelitis optica is characterized by ON, which is often bilateral (simultaneously or sequentially), and longitudinally extensive TM with a well-defined sensory level, as well as sphincter dysfunction, pain and tonic spasms of the trunk and extremities. Involvement of the brain stem may cause hiccups, nausea and even respiratory failure, while hypothalamic-pituitary axis dysfunction commonly manifests as hyponatremia, hyperthermia and hyperprolactinemia. Encephalopathy mimicking posterior reversible encephalopathy syndrome has also been described.
Clinical attacks generally progress over days, with variable recovery within months. Most patients endure some residual disability, which accumulates over time.
Even though a review of the complicated mechanisms of pathogenesis of NMO is far from the purpose of our review, in order to explore in which ways HLA may contribute to it, we refer to some important aspects, providing the basics for this purpose. AQP4-antibodies have a decisive role in the pathogenesis of NMO, by complement-mediated astrocyte damage, cascading to leukocyte infiltration, oligodendrocyte death and neuronal cell damage. They are present in up to 80% of NMO cases. AQP4 is highly expressed in astrocytic end-feet in the blood-brain barrier, nodes of Ranvier and neuronal synapses. AQP4 is also expressed in a sub-population of CNS ependymal cells associated with the pia, subfornical organ and to a lesser extent in other ependymal cells (not in the choroid plexus), in situ in lipopolysaccharide-activated microglia and on retinal astrocytes (Müller cells). It is abundant in the grey matter of the spinal cord, the periventricular and periaqueductal area. Outside the CNS, it is found on epithelial cells of the kidney collecting ducts, airways, parietal cells of the stomach, skeletal muscle sarcolemma and colon. AQP4-IgG serum levels are found to correlate with NMO disease activity, distinct phenotypic features (gender, course and co-existing autoimmunity), severity and response to treatment. In patients with isolated ON or isolated longitudinally extensive TM, AQP4-antibodies have been shown to predict conversion to NMO. Recent researches have shown that patients’ sera with MS, acute disseminated encephalomyelitis, systemic lupus erythematosus (SLE) and Sjögren syndrome (SS) were negative for AQP4-antibodies.[28,29] However, Alexopoulos et al. managed to demonstrate that, despite the negativity of the serum in antibodies, 13% of the sera with relapsing-remitting MS reacted with the epitope AQPaa252-275 (NMO-positive sera exhibited reactivity against the intracellular epitope AQPaa252-275 in this study, confirming previous observations).
Additional immunological components participate. NMO lesions contain large numbers of macrophages, eosinophils and neutrophils, on which AQP4-IgG acts by binding to Fc receptors, as well as on B-cells, which produce interleukin (IL)-6. IL-17 and IL-6 are the main pro-inflammatory cytokines which are found to be elevated in the serum and cerebro-spinal fluid of patients with NMO. T-cells, though fewer, are also certainly relevant, as T-helper cells are involved in B-cell isotype switching and affinity maturation. The possible role of natural killer cells and glutamate- mediated excitotoxicity has also been discussed. Of course, it has become clear that NMO is associated with certain HLA alleles, which are extensively described below.
There is a strong association between NMO and other autoimmune diseases, especially in NMO-IgG positive patients: co-existence with autoimmune thyroid disease, SLE, SS, celiac disease, sarcoidosis, or myasthenia gravis (MG) has been described in a higher frequency than it could be by chance. This co-association could be due to common genetic factors, such as HLA and non-HLA genes, including PTPN22, a tyrosine phosphatase associated with type-1 diabetes, rheumatoid arthritis (RA), SLE, Crohn’s disease and MG;[31,32]IL-23R, associated with SLE, Crohn’s disease and psoriasis; and finally, TNFAIP3, involved in control of unbiquitination, associated with RA, SLE, Crohn’s disease and psoriasis. As far as MG is concerned, despite of the well-established link between the thymus gland and MG (human thymus tissue has been shown to express AChR, which is widely thought to be a triggering mechanism in early-onset AChR-MG), recent evidence suggests that AQP4 is also expressed in human thymus suggesting a similar and early involvement of the thymus in NMO-spectrum diseases (NMOSD).[33-36] HLA-DRB1*03 and especially the whole haplotype, HLA A1-B8-DR3-DQ2, is the most commonly attributed to MG in the Caucasians, an haplotype common and in other autoimmune diseases. HLA-DRB1*03 allele is highly frequent in the NMO-IgG positive patients, as we explain in detail below and it could be one of the links between MG and NMO.
According to the results of the researches that have been conducted in Japanese, conventional MS is associated with the HLA-DRB1*15:01 allele,[12,38,39] while OSMS, which is now accepted as a component of the NMO spectrum, is associated with the HLA-DPB1*05:01.[40-45] HLA-DPB1*05:01 is the most common DPB1 allele in Japanese, which may explain the frequent occurrence of anti-AQP4 antibody in Japanese OSMS. Nevertheless, Fukazawa et al. in 2006 came to the conclusion that HLA-DPB1*05:01 plays an important role in the development of MS in general, but not in OSMS. The strong association of HLA-DPB1*05:01 with OSMS may be due to the over-representation of the HLA-DPB1*03:01 allele among individuals in the non-OSMS group, a question that needs further investigation. The observed protective effect of HLA- DRB1*01 in anti-AQP4-negative MS patients is in accordance with findings in Caucasians.[47,48]
It is also estimated that there is a protective effect of HLA-DRB1*09 in anti-AQP4-negative MS patients, probably by reducing the susceptibility attributed to HLA-DRB1*15, as individuals with a HLA- DRB1*09/HLA-DRB1*15 genotype have a decreased risk of anti-AQP4-negative MS. Recently, HLA- DRB1*09 was also shown to be negatively associated with ulcerative colitis. Thus, it is assumed that HLA- DRB1*09, or some genes in linkage disequilibrium with it, protect against certain autoimmune diseases, at least in Japanese, as it is quite rare in Caucasians. Moreover, a predisposing effect of HLA-DRB1*12 in anti-AQP4-positive MS has been found. Interestingly, HLA-DRB1*12 has been reported to increase the risk of allergic disorders, such as asthma, urticaria, and food allergy. Finally, HLA-DRB1*04/HLA-DRB1*04, HLA-DRB1*04/HLA-DRB1*14, and HLA-DRB1*04/HLA-DRB1*15 genotypes increase the risk of non-NMO MS, probably by interaction with DRB1*15 allele.
In contrast, researches in Caucasian populations have come to the conclusion that the HLA-DRB1*03 allele is highly frequent in the NMO-IgG positive patients, while DPB1*05:01 is quite rare both in patients and healthy controls. We should also highlight the negative association between HLA-DRB1*15:01 and NMO, which indicates a possible protective role.[53,54] In this point, the observation that NMO-IgG- positive and negative patients differ mostly in terms of gender and the association of other autoimmune diseases, could imply that HLA-DRB1*03 is associated with the NMO-IgG presence, but not with NMO per se and raise the question of whether NMO-IgG is epiphenomenon or pathogenic. In reply to this, Arellano et al. found that hAQP4281-330 is the dominant linear immunogenic determinant of hAQP4 in the context of HLADRB1*03:01. Within hAQP4281- 330 are two dominant immunogenic determinants that induce differential Th phenotypes. In recent times, Asgari et al.[27,53] reported a high frequency of HLA- DQB1*04:02 in NMO patients, an allele described to be associated with autoimmune diseases such as primary biliary cirrhosis, type-1 diabetes and juvenile idiopathic arthritis, but he didn’t show any correlation with HLA-DRB1*03.
In Brazilian cohorts, NMO patients present a high frequency of the HLA-DRB1*03 allele and extremely low frequency of the HLA-DRB1*15. In addition to this, the same study showed that HLA- DRB1*01 allele is associated with NMO and benign MS, a correlation that indicates that this allele may influence the outcome of these demyelinating disorders. We would like to emphasize once more that HLA-DRB1*01 has a protective effect in anti-AQP4-negative MS patients in Japanese and Caucasians.[47,48] In African-Americans, none OSMS patient carries the HLA-DRB1*1501 allele, while in Afro-Carribeans, NMO has been associated with the HLA-DRB1*03 allele [Table 2].
The distribution of HLA alleles in different ethnic groups
|Caucasians||HLA class I||No correlations found|||
|HLA-DR||HLA-DRB1*01||High frequency in NMO-IgG positive patients|||
|HLA-DRB1*0301||High frequency in NMO-IgG positive patients||[27,37,53]|
|Not demonstrated association|
|HLA-DRB1*1501||Not associated with NMO||[37,54]|
|HLA-DQ||HLA-DQB1*0402||Higher frequency in NMO compared to HCs||[27,53]|
|Increased in NMO|
|HLA-DQA1*0102||High frequency in NMO-IgG negative patients||[37,53]|
|No significant differences noticed|
|HLA-DP||HLA-DPB1*0501||Rare allele in Caucasians. No correlations found|||
|Japanese-Chinese||HLA class I||No data||No data|
|HLA-DR||HLA-DRB1*01||Protective effect on anti-AQP4 negative MS patients|||
|HLA-DRB1*04||Increases the risk of non-NMO MS, especially|||
|HLA-DRB1*04/04, 04/14, 04/15|
|HLA-DRB1*09||Protective factor for anti-AQP4 negative MS, especially HLA-DRB1*09/15. Decreased the risk of anti-AQP4 positive MS in monovariate studies||[39,40]|
|NMO/NMOSD patients showed a significantly lower frequency|
|HLA-DRB1*12||Increased frequency in anti-AQP4 positive MS, especially HLA-DRB1*12/15|||
|HLA-DRB1*15||Common in MS patients. Probable in correlation with *04, *09, *12 alleles||[12,38,39]|
|Association with common MS|
|HLA-DRB1*1602||Higher frequency in anti-AQP4 positive patients in Han Chinese||[40,41]|
|Risk factor only for anti-AQP4 positive NMO/NMOSD|
|HLA-DP||HLA-DPB1*0501||Strong positive association with OSMS||[12,38,40-46]|
|Associated with opticospinal MS|
|Increased frequency in anti-AQP4 positive patients|
|Risk factor only for anti-AQP4 positive NMO/NMOSD patients|
|Susceptibility in anti-AQP4 positive NMO in Han Chinese|
|Important role in the development of MS in general, but not in OSMS. The strong association of DPB1*0501 with OSMS may be due to the over-representation of the DPB1*0301 allele among individuals in the non-OSMS|
|HLA-DPB1*0301||The most strongly associated allele with conventional MS, complete lack in OSMS||[38,44]|
|Possible protection against the development of OSMS|
|Brazilians||HLA-class I||No data||No data|
|HLA-DR||HLA-DRB1*01||High frequency in NMO|||
|HLA-DRB1*03||High frequency in NMO|||
|HLA-DRB1*15||Low frequency in NMO, possible protective role|||
|African-Americans and Afro-Caribbeans||HLA class I||No data||No data|
|HLA-DR||HLA-DRB1*1501||None OSMS African-American patient||[57,58]|
|HLA-DRB1*03||Highly noticed in NMO Afro-Caribbean patients|
To the best of our knowledge, this is the first review aiming at summarizing all the results concerning HLA allelic frequencies in NMO and NMOSD, worldwide. Apart from a detailed description of HLA allelic frequencies in all genotyped NMO ethnic groups, we created a workable table including all this information, for an easier reader’s approach.
As a conclusion, it is clear that quite different HLA- alleles are correlated to NMO/NMOSD compared to MS patients, reflecting different underlying immunopathogenic mechanisms. Particularly, the well- established and most frequent HLA-DRB1*15:01 allele, associated with MS, plays rather a protective role for NMO. In addition, rare alleles, HLA-DRB1*12, like HLA-DRB1*01 and especially HLA-DRB1*09, play a core role in NMO risk or protection respectively and obviously in immonopathogenesis, in some ethnic groups. On the other hand, it is clear that different HLA alleles are associated with different ethnic groups, like Eastern NMO (association with the HLA-DPB1*05:01), which in turn are specifically associated with certain clinical/paraclinical features.
Moreover, the comorbidity of NMO with other autoimmune diseases is still under further investigation, although it seems that so far this comorbidity is highly reflected in HLA profile and anti-AQP4 antibody presence, suggesting common pathways in their immunopathogenesis.
In MS there is also comorbidity with other autoimmune diseases, like SLE, Hashimoto’s thyroiditis, etc., However, this co-existence presumes rarer than in NMO, although more investigation studies are warranted to prove this notion.
In this paper, we tried to focus only on the HLA- immunogenetics of NMO, since the HLA molecule is a core component of the trimolecular complex, which is involved in antigen-presentation, as the first step of the immune response. However, as in MS, similarly in NMO, many non-MHC genes are candidates for the overall genetic burden. First, genes correlated to immune system and immunogenetics, namely IL-7 receptor polymorphisms, IL-2 receptor a chain gene polymorphisms and CD6, interferon regulatory factor 8 and tumor necrosis factor receptor superfamily polymorphisms and secondly, the polymorphisms of the promoter region of cytochrome-P450-7A1 gene[62,63] and AQP4 genetic variations are involved.
Regarding MS, it has been shown that specific alleles, in particular HLA-DRB1*04:01, HLA-DRB1*04:08 and HLA-DRB1*16:01, are associated with an increased risk of antiinterferon beta antibody development. As a result, the poorer therapeutical outcome highligths the importance of the stratification of patients to responders and nonresponders, according to HLA- genotyping. Similarly, Warabi et al. concluded that patients carrying the NMO-specific HLA allele DPB1*05:01 showed a poor prognosis following interferon beta-1b treatment. The crucial role of the AQP4-antibodies in the pathogenesis of NMO has been in our consideration for a few years only, in contrast to the 30 years of worldwide research regarding MS and HLA. We expect this to be the new field of extensive future research, in correlation with the accumulated knowledge on the pathogenesis of NMO.
Finally, the HLA profile in a patient with a CNS demyelinating disease tends to highlight different backgrounds in immunopathogenesis and clinical phenotype, components which are very important in the diagnosis and disease therapeutic decision making, which is strongly requested. To this direction and in order to use HLA alleles, as a biomarker, in patients’ early stratification, more HLA-genotyping studies are needed, in different ethnic groups, in order to clarify, replicate or even expand the already existed results.
There are no conflicts of interest.
1. Katsavos S, Anagnostouli M. Biomarkers in multiple sclerosis: an up-to-date overview. Mult Scler Int 2013;2013:340508.
2. Ramagopalan SV, Maugeri NJ, Handunnetthi L, Lincoln MR, Orton SM, Dyment DA, Deluca GC, Herrera BM, Chao MJ, Sadovnick AD, Ebers GC, Knight JC. Expression of the multiple sclerosis-associated MHC class II Allele HLA-DRB1*1501 is regulated by vitamin D. PLoS Genet 2009;5:e1000369.DOIPubMedPMC
3. Anagnostouli M, Anagnostoulis G, Katsavos S, Panagiotou M, Kararizou E, Davaki P. HLA-DRB1 15:01 and Epstein-Barr virus in a multiple sclerosis patient with psoriasis, nasopharyngeal and breast cancers. Lessons for possible hidden links for autoimmunity and cancer. J Neurol Sci 2014;339:26-31.DOIPubMed
4. Kikuchi S, Fukazawa T, Niino M, Yabe I, Miyagishi R, Hamada T, Tashiro K. Estrogen receptor gene polymorphism and multiple sclerosis in Japanese patients: interaction with HLA-DRB1*1501 and disease modulation. J Neuroimmunol 2002;128:77-81.DOI
5. Cree BA, Goodin DS, Hauser SL. Neuromyelitis optica. Semin Neurol 2002;22:105-22.DOIPubMed
6. Lennon VA, Wingerchuk DM, Kryzer TJ, Pittock SJ, Lucchinetti CF, Fujihara K, Nakashima I, Weinshenker BG. A serum autoantibody marker of neuromyelitis optica: distinction from multiple sclerosis. Lancet 2004;364:2106-12.DOI
7. Devic E. Myelite subaigue compliquee de nevrite optique. Bull Med (Paris) 1894;8:1033-4.
8. Wingerchuk DM, Hogancamp WF, O'Brien PC, Weinshenker BG. The clinical course of neuromyelitis optica (Devic's syndrome). Neurology 1999;53:1107-14.DOIPubMed
9. Wingerchuk DM, Lennon VA, Pittock SJ, Lucchinetti CF, Weinshenker BG. Revised diagnostic criteria for neuromyelitis optica. Neurology 2006;66:1485-9.DOIPubMed
10. Cree BA, Khan O, Bourdette D, Goodin DS, Cohen JA, Marrie RA, Glidden D, Weinstock-Guttman B, Reich D, Patterson N, Haines JL, Pericak-Vance M, DeLoa C, Oksenberg JR, Hauser SL. Clinical characteristics of African Americans vs Caucasian Americans with multiple sclerosis. Neurology 2004;63:2039-45.DOIPubMed
11. Cabre P, Heinzlef O, Merle H, Buisson GG, Bera O, Bellance R, Vernant JC, Smadja D. MS and neuromyelitis optica in Martinique (French West Indies). Neurology 2001;56:507-14.DOIPubMed
12. Kira J. Multiple sclerosis in the Japanese population. Lancet Neurol 2003;2:117-27.DOI
13. Das A, Puvanendran K. A retrospective review of patients with clinically definite multiple sclerosis. Ann Acad Med Singapore 1998;27:204-9.PubMed
14. Gangopadhyay G, Das SK, Sarda P, Saha SP, Gangopadhyay PK, Roy TN, Maity B. Clinical profile of multiple sclerosis in Bengal. Neurol India 1999;47:18-21.PubMed
15. Papais-Alvarenga RM, Miranda-Santos CM, Puccioni-Sohler M, de Almeida AM, Oliveira S, Basilio De Oliveira CA, Alvarenga H, Poser CM. Optic neuromyelitis syndrome in Brazilian patients. J Neurol Neurosurg Psychiatry 2002;73:429-35.DOIPubMedPMC
16. Misu T, Fujihara K, Nakashima I, Miyazawa I, Okita N, Takase S, Itoyama Y. Pure optic-spinal form of multiple sclerosis in Japan. Brain 2002;125:2460-8.DOIPubMed
17. de Seze J, Lebrun C, Stojkovic T, Ferriby D, Chatel M, Vermersch P. Is Devic's neuromyelitis optica a separate disease? A comparative study with multiple sclerosis. Mult Scler 2003;9:521-5.DOIPubMed
18. Ghezzi A, Bergamaschi R, Martinelli V, Trojano M, Tola MR, Merelli E, Mancardi L, Gallo P, Filippi M, Zaffaroni M, Comi G; Italian Devic's Study Group (IDESG). Clinical characteristics, course and prognosis of relapsing Devic's neuromyelitis optica. J Neurol 2004;251:47-52.DOIPubMed
19. Davis R, Thiele E, Barnes P, Riviello JJ, Jr. Neuromyelitis optica in childhood: case report with sequential MRI findings. J Child Neurol 1996;11:164-7.DOIPubMed
20. Filley CM, Sternberg PE, Norenberg MD. Neuromyelitis optica in the elderly. Arch Neurol 1984;41:670-2.DOIPubMed
21. Matiello M, Kim HJ, Kim W, Brum DG, Barreira AA, Kingsbury DJ, Plant GT, Adoni T, Weinshenker BG. Familial neuromyelitis optica. Neurology 2010;75:310-5.DOIPubMedPMC
22. Misu T, Fujihara K, Nakashima I, Sato S, Itoyama Y. Intractable hiccup and nausea with periaqueductal lesions in neuromyelitis optica. Neurology 2005;65:1479-82.DOIPubMed
23. Poppe AY, Lapierre Y, Melançon D, Lowden D, Wardell L, Fullerton LM, Bar-Or A. Neuromyelitis optica with hypothalamic involvement. Mult Scler 2005;11:617-21.DOIPubMed
24. Maga-a SM, Matiello M, Pittock SJ, McKeon A, Lennon VA, Rabinstein AA, Shuster E, K antarci OH, Lucchinetti CF, Weinshenker BG. Posterior reversible encephalopathy syndrome in neuromyelitis optica spectrum disorders. Neurology 2009;72:712-7.DOIPubMed
25. Jarius S, Wildemann B, Paul F. Neuromyelitis optica: clinical features, immunopathogenesis and treatment. Clin Exp Immunol 2014;176:149-64.DOIPubMedPMC
26. Graber DJ, Levy M, Kerr D, Wade WF. Neuromyelitis optica pathogenesis and aquaporin 4. J Neuroinflammation 2008;5:22.DOIPubMedPMC
27. Asgari N. Epidemiological, clinical and immunological aspects of neuromyelitis optica (NMO). Dan Med J 2013;60:B4730.PubMed
28. Alexopoulos H, K ampylafka EI, Chatzi I, Travasarou M, Karageorgiou KE, Dalakas MC, Tzioufas AG. Reactivity to AQP4 epitopes in relapsing-remitting multiple sclerosis. J Neuroimmunol 2013;260:117-20.DOIPubMed
29. Mader S, Gredler V, Schanda K, Rostasy K, Dujmovic I, Pfaller K, Lutterotti A, Jarius S, Di Pauli F, Kuenz B, Ehling R, Hegen H, Deisenhammer F, Aboul-Enein F, Storch MK, Koson P, Drulovic J, Kristoferitsch W, Berger T, Reindl M. Complement activating antibodies to myelin oligodendrocyte glycoprotein in neuromyelitis optica and related disorders. J Neuroinflammation 2011;8:184.DOIPubMedPMC
30. Iyer A, Elsone L, Appleton R, Jacob A. A review of the current literature and a guide to the early diagnosis of autoimmune disorders associated with neuromyelitis optica. Autoimmunity 2014;47:154-61.DOIPubMed
31. Wingerchuk DM, Weinshenker BG. The emerging relationship between neuromyelitis optica and systemic rheumatologic autoimmune disease. Mult Scler 2012;18:5-10.DOIPubMed
32. Avidan N, Le Panse R, Berrih-Aknin S, Miller A. Genetic basis of myasthenia gravis - a comprehensive review. J Autoimmun 2013;52:146-53.DOIPubMed
33. Schluep M, Willcox N, Vincent A, Dhoot GK, Newsom-Davis J. Acetylcholine receptors in human thymic myoid cells in situ: an immunohistological study. Ann Neurol 1987;22:212-22.DOIPubMed
34. Wekerle H, Ketelsen UP. Intrathymic pathogenesis and dual genetic control of myasthenia gravis. Lancet 1977;1:678-80.DOI
35. Leite MI, Jones M, Ströbel P, Marx A, Gold R, Niks E, Verschuuren JJ, Berrih-Aknin S, Scaravilli F, Canelhas A, Morgan BP, Vincent A, Willcox N. Myasthenia gravis thymus: complement vulnerability of epithelial and myoid cells, complement attack on them, and correlations with autoantibody status. Am J Pathol 2007;171:893-905.DOIPubMedPMC
36. Giraud M, Taubert R, Vandiedonck C, Ke X, Lévi-Strauss M, Pagani F, Baralle FE, Eymard B, Tranchant C, Gajdos P, Vincent A, Willcox N, Beeson D, Kyewski B, Garchon HJ. An IRF8-binding promoter variant and AIRE control CHRNA1 promiscuous expression in thymus. Nature 2007;448:934-7.DOIPubMed
37. Zéphir H, Fajardy I, Outteryck O, Blanc F, Roger N, Fleury M, Rudolf G, Marignier R, Vukusic S, Confavreux C, Vermersch P, de Seze J. Is neuromyelitis optica associated with human leukocyte antigen? Mult Scler 2009;15:571-9.DOIPubMed
38. Fukazawa T, Yamasaki K, Ito H, Kikuchi S, Minohara M, Horiuchi I, Tsukishima E, Sasaki H, Hamada T, Nishimura Y, Tashiro K, Kira J. Both the HLA-CPB1 and -DRB1 alleles correlate with risk for multiple sclerosis in Japanese: clinical phenotypes and gender as important factors. Tissue Antigens 2000;55:199-205.DOIPubMed
39. Isobe N, Matsushita T, Yamasaki R, Ramagopalan SV, Kawano Y, Nishimura Y, Ebers GC, Kira J. Influence of HLA-DRB1 alleles on the susceptibility and resistance to multiple sclerosis in Japanese patients with respect to anti-aquaporin 4 antibody status. Mult Scler 2010;16:147-55.DOIPubMed
40. Yoshimura S, Isobe N, Matsushita T, Yonekawa T, Masaki K, Sato S, Kawano Y, Kira J; South Japan Multiple Sclerosis Genetics Consortium. Distinct genetic and infectious profiles in Japanese neuromyelitis optica patients according to anti-aquaporin 4 antibody status. J Neurol Neurosurg Psychiatry 2013;84:29-34.DOIPubMed
41. Wang H, Dai Y, Qiu W, Zhong X, Wu A, Wang Y, Lu Z, Bao J, Hu X. HLA-DPB1*0501 is associated with susceptibility to anti-aquaporin-4 antibodies positive neuromyelitis optica in southern Han Chinese. J Neuroimmunol 2011;233:181-4.DOIPubMed
42. Matsushita T, Matsuoka T, Isobe N, Kawano Y, Minohara M, Shi N, Nishimura Y, Ochi H, Kira J. Association of the HLA-DPB1*0501 allele with anti-aquaporin-4 antibody positivity in Japanese patients with idiopathic central nervous system demyelinating disorders. Tissue Antigens 2009;73:171-6.DOIPubMed
43. Kira J. Neuromyelitis optica and opticospinal multiple sclerosis: mechanisms and pathogenesis. Pathophysiology 2011;18:69-79.DOIPubMed
44. Fukazawa T, Kikuchi S, Miyagishi R, Miyazaki Y, Yabe I, Hamada T, Sasaki H. HLA-dPB1*0501 is not uniquely associated with opticospinal multiple sclerosis in Japanese patients. Important role of DPB1 * 0301. Mult Scler 2006;12:19-23.DOIPubMed
45. Ito H, Yamasaki K, Kawano Y, Horiuchi I, Yun C, Nishimura Y, Kira J. HLA-DP-associated susceptibility to the optico-spinal form of multiple sclerosis in the Japanese. Tissue Antigens 1998;52:179-82.DOIPubMed
46. Yamasaki K, Horiuchi I, Minohara M, Kawano Y, Ohyagi Y, Yamada T, Mihara F, Ito H, Nishimura Y, Kira J. HLA-DPB1 * 0501-associated opticospinal multiple sclerosis: clinical, neuroimaging and immunogenetic studies. Brain 1999;122:1689-96.DOIPubMed
47. Ramagopalan SV, Morris AP, Dyment DA, Herrera BM, DeLuca GC, Lincoln MR, Orton SM, Chao MJ, Sadovnick AD, Ebers GC. The inheritance of resistance alleles in multiple sclerosis. PLoS Genet 2007;3:1607-13.DOIPubMedPMC
48. DeLuca GC, Ramagopalan SV, Herrera BM, Dyment DA, Lincoln MR, Montpetit A, Pugliatti M, Barnardo MC, Risch NJ, Sadovnick AD, Chao M, Sotgiu S, Hudson TJ, Ebers GC. An extremes of outcome strategy provides evidence that multiple sclerosis severity is determined by alleles at the HLA-DRB1 locus. Proc Natl Acad Sci U S A 2007;104:20896-901.DOIPubMedPMC
49. Mochida A, Kinouchi Y, Negoro K, Takahashi S, Takagi S, Nomura E, Kakuta Y, Tosa M, Shimosegawa T. Butyrophilin-like 2 gene is associated with ulcerative colitis in the Japanese under strong linkage disequilibrium with HLA-DRB1*1502. Tissue Antigens 2007;70:128-35.DOIPubMed
50. Movahedi M, Moin M, Gharagozlou M, Aghamohammadi A, Dianat S, Moradi B, Nicknam MH, Nikbin B, Amirzargar A. Association of HLA class II alleles with childhood asthma and Total IgE levels. Iran J Allergy Asthma Immunol 2008;7:215-20.PubMed
51. Chen J, Tan Z, Li J, Xiong P. Association of HLA-DRB1, DQB1 alleles with chronic urticaria. J Huazhong Univ Sci Technolog Med Sci 2005;25:354-6.DOIPubMed
52. Boehncke WH, Loeliger C, Kuehnl P, Kalbacher H, Böhm BO, Gall H. Identification of HLA-DR and -DQ alleles conferring susceptibility to pollen allergy and pollen associated food allergy. Clin Exp Allergy 1998;28:434-41.DOIPubMed
53. Asgari N, Nielsen C, Stenager E, Kyvik KO, Lillevang ST. HLA, PTPN22 and PD-1 associations as markers of autoimmunity in neuromyelitis optica. Mult Scler 2012;18:23-30.DOIPubMed
54. Matiello M, Schaefer-Klein J, Brum DG, Atkinson EJ, Kantarci OH, Weinshenker BG; NMO genetics collaborators. HLA-DRB1*1501 tagging rs3135388 polymorphism is not associated with neuromyelitis optica. Mult Scler 2010;16:981-4.DOIPubMed
55. Arellano B, Hussain R, Zacharias T, Yoon J, David C, Zein S, Steinman L, Forsthuber T, Greenberg BM, Lambracht-Washington D, Ritchie AM, Bennett JL, Stüve O. Human aquaporin 4281-300 is the immunodominant linear determinant in the context of HLA-DRB1*03:01: relevance for diagnosing and monitoring patients with neuromyelitis optica. Arch Neurol 2012;69:1125-31.DOIPubMedPMC
56. Brum DG, Barreira AA, dos Santos AC, Kaimen-Maciel DR, Matiello M, Costa RM, Deghaide NH, Costa LS, Louzada-Junior P, Diniz PR, Comini-Frota ER, Mendes-Junior CT, Donadi EA. HLA-DRB association in neuromyelitis optica is different from that observed in multiple sclerosis. Mult Scler 2010;16:21-9.DOIPubMed
57. Cree BA, Reich DE, Khan O, De Jager PL, Nakashima I, Takahashi T, Bar-Or A, Tong C, Hauser SL, Oksenberg JR. Modification of Multiple Sclerosis Phenotypes by African Ancestry at HLA. Arch Neurol 2009;66:226-33.DOIPubMedPMC
58. Deschamps R, Paturel L, Jeannin S, Chausson N, Olindo S, Béra O, Bellance R, Smadja D, Césaire D, Cabre P. Different HLA class II (DRB1 and DQB1) alleles determine either susceptibility or resistance to NMO and multiple sclerosis among the French Afro-Caribbean population. Mult Scler 2011;17:24-31.DOIPubMed
59. Kim JY, Cheong HS, Kim HJ, Kim LH, Namgoong S, Shin HD. Association analysis of IL7R polymorphisms with inflammatory demyelinating diseases. Mol Med Rep 2014;9:737-43.DOIPubMed
60. Ainiding G, Kawano Y, Sato S, Isobe N, Matsushita T, Yoshimura S, Yonekawa T, Yamasaki R, Murai H, Kira J, South Japan Multiple Sclerosis Genetics Consortium. Interleukin 2 receptor α chain gene polymorphisms and risks of multiple sclerosis and neuromyelitis optica in southern Japanese. J Neurol Sci 2014;337:147-50.DOIPubMed
61. Park TJ, Kim HJ, Kim JH, Bae JS, Cheong HS, Park BL, Shin HD. Associations of CD6, TNFRSF1A and IRF8 polymorphisms with risk of inflammatory demyelinating diseases. Neuropathol Appl Neurobiol 2013;39:519-30.DOIPubMed
62. Zhao GX, Liu Y, Li ZX, Lv CZ, Traboulsee A, Sadovnick AD, Wu ZY. Variants in the promoter region of CYP7A1 are associated with neuromyelitis optica but not with multiple sclerosis in the Han Chinese population. Neurosci Bull 2013;29:525-30.DOIPubMedPMC
63. Kim HJ, Park HY, Kim E, Lee KS, Kim KK, Choi BO, Kim SM, Bae JS, Lee SO, Chun JY, Park TJ, Cheong HS, Jo I, Shin HD. Common CYP7A1 promoter polymorphism associated with risk of neuromyelitis optica. Neurobiol Dis 2010;37:349-55.DOIPubMed
64. Matiello M, Schaefer-Klein JL, Hebrink DD, Kingsbury DJ, Atkinson EJ, Weinshenker BG; NMO Genetics Collaborators. Genetic analysis of aquaporin-4 in neuromyelitis optica. Neurology 2011;77:1149-55.DOIPubMed
65. Buck D, Cepok S, Hoffmann S, Grummel V, Jochim A, Berthele A, Hartung HP, Wassmuth R, Hemmer B. Influence of the HLA-DRB1 genotype on antibody development to interferon beta in multiple sclerosis. Arch Neurol 2011;68:480-7.DOIPubMed
66. Warabi Y, Matsumoto Y, Hayashi H. Interferon beta-1b exacerbates multiple sclerosis with severe optic nerve and spinal cord demyelination. J Neurol Sci 2007;252:57-61.DOIPubMed
Lu Zhang et al., Neuroimmunology and Neuroinflammation, 2019
Cong Zhao et al., Neuroimmunology and Neuroinflammation, 2016