fig03

Figure 3. LPS-induced TNFα gene expression. A: CHME-5 cells were treated with LPS at 37 °C for 3, 6, and 18 h. TNFα mRNA expression was analyzed by RT-PCR analysis with β-actin as housekeeping gene, 3 h (**P < 0.002), 6 h (*P < 0.02), and 18 h (**P < 0.003) vs. US. Image is representative of 3 independent experiments (n = 3) for each treatment group; B: cell viability absorbance was read at 492 nm, *P < 0.01 vs. US. Experiments were carried out in duplicate (n = 3) for each treatment group. Bars for all groups are presented as mean ± SEM. LPS: lipopolysaccharide; TNFα: tumor necrosis factor-α; RT-PCR: real-time polymerase chain reaction; US: unstimulated