fig2

Supplementary Figure 2. α-tubulin and histone 3-immunoreactivity in CHME-5 cellular fractions. CHME-5 cells were sitmulated with LPS (1 μg/mL) and incubated at 37 °C for a time course (0-270 min). A: cytoplasmic and nuclear extracts were subjected to SDS-PAGE electrophoresis and immunoblotted with α-tubulin (1:1,000) or histone 3 (1:2,000) antibodies; B: integrated of α-tubulin cytoplasmic fractions, (**P = 0.003, ***P = 0.0004, ****P < 0.0001); C: similarly, histone 3-immunoreactivity was significantly increased in nuclear fractions, (****P < 0.0001). Image is representative of 3 individual experiments, n = 3. Data points are given as mean ± SEM. LPS: lipopolysaccharide; SDS-PAGE： sodium dodecyl sulfate polyacrylamide gel electrophoresi