fig6

Figure 6. Quantitative analysis of CD68 and TLR4 immunofluorescence. CHME-5 cells were stimulated with LPS (1 μg/mL) at 37 °C for 10 min. Cells were fixed and stained. A: CD68 (1:1,000) and anti-rabbit-Alexa Fluor 647 (1:1,000); B: TLR4 (1:1,000) and anti-mouse-Alexa Fluor 555 (1:1,000) antibodies; A-B: epifluorescence microscopy-40× objective; C-D: CellProfiler analysis for mean grey intensity, (****P < 0.0001) vs. NEG and (#P < 0.0001) vs. US. Bars are presented as mean ± SEM. CD68: cluster of differentiation 68; TLR4: toll-like receptor 4; LPS: lipopolysaccharide; NEG: negative control (only incubated with secondary antibody not primary), US: unstimulated