fig7

Figure 7. Visualization and 3D reconstruction of CD68 and TLR4 immunofluorescence. CHME-5 cells were stimulated with LPS (1 μg/mL) at 37 °C for 10 min. A: brightfield images were captured with 100× oil objective, cells were fixed and labeled; B: TLR4 (1:1,000) and anti-mouse-Alexa Fluor 555 (1:1,000); C: CD68 (1:1,000) and anti-rabbit-Alexa Fluor 647 (1:1,000) antibodies and DAPI (blue); B-D: confocal microscopy-100× oil objective; D: images merged with DAPI; E: 3D reconstruction with overlay on brightfield images; F: 3D reconstruction of close-up side view. CD68: cluster of differentiation 68; TLR4: toll-like receptor 4; LPS: lipopolysaccharide; DAPI: 4'-6-diamidino-2-phenylindole