fig3

Figure 3. TREM2 expression by microglia in AD brain tissue sections. Immunohistochemical localization of TREM2 protein using antibody AF1828 (R&D systems). Sections from an AD case are shown: (A) single-stained section showing microglial-like structures immunoreactive for TREM2. Purple represents reaction with nickel-enhanced diaminobenzidine substrate; (B) double-staining (TREM2 - purple - P2RY12- brown) of AD section showing colocalization of TREM2 and P2RY12. Arrows indicate TREM2 positive microglia (purple) adjacent to P2RY12 microglia